Other

Part:BBa_K277079:Design

Designed by: James DiCarlo   Group: iGEM09_Johns_Hopkins-BAG   (2009-10-21)


3L.3_23.C2.09


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 348
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 348
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 348
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 348
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 348
    Illegal AgeI site found at 6
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 369


Design Notes

Constructed using overlap extension PCR protocol described in RFC38


Source

Synthetic Yeast

References